The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assays, resulting in false negatives or . objetivos: conocer las diferentes técnicas de aglutinación usadas en el laboratorio. adquirir habilidad y destrezas en las técnicas de. Prozone effect: No prozone effect was detected upon mg/dL. 4. . Efecto prozona: No se observa efecto prozona hasta valores de
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In this case, free antigen is in competition with captured antigen for detection antibody binding.
Views Read Edit View history. In this case, the result is a false negative.
The range of relatively high antibody concentrations within which no reaction occurs is called the prozone. Because no agglutination efecyo, the test is interpreted as negative.
Retrieved 3 February The serological test is mainly seen in the precipitation reaction. The antibody that fails to react is known as the blocking antibody and prevents the precipitating antibody from binding to the antigens.
The effect can also occur because of antigen excess, when both the capture and detection antibodies become saturated by the high analyte concentration.
In this case, no sandwich can be formed by the capturing antibody, the antigen and the detection antibody.
Hook effect – Wikipedia
However, if too many antibodies are present that can bind to the antigen, then the antigenic sites are coated by antibodies, and few or no antibodies directed toward the pathogen are able to prozina more than one antigenic particle. The phenomenon is caused by very high concentrations of a particular analyte or antibody and is most prevalent in one-step sandwich immunoassays.
The hook effect or the prozone effect is a type of interference which plagues proznoa immunoassays and nephelometric assaysresulting in false negatives or inaccurately low results. Examples include high levels of syphilis antibodies in HIV patients or high levels of cryptococcal antigen leading to false negative tests in undiluted samples.
The Clinical Biochemist Reviews. Other common forms of interference include antibody interference, cross-reactivity and signal interference. Retrieved from ” https: If the antibodies agglutinate with the antigen to form immune complexesefectto the test is interpreted as positive.
Term Bank – efecto prozona – Spanish English Dictionary
Thus the proper precipitation reaction does not take place.